What is recombinant protein? Proteins are one of the most important biological molecules for life. And these proteins perform a vast array of functions within organisms, including catalysing metabolic reactions, DNA replication, responding to stimuli and transporting molecules from one location to another. However, when we want to analyze the structure, function, mechanism, pathway or other facts of these proteins, we will find that the quantities are not sufficient from nature source. In that case we need to produce high quantity and purity proteins in a short time and low cost in our lab. Thus, recombinant protein technology is required. Recombinant protein is a manipulated form of protein, which is generated in various ways to produce large quantities of proteins, modify gene sequences and manufacture useful commercial products. Recombinant protein is encoded by recombinant DNA, which has been cloned in a system that supports expression of the gene and translation of mRNA. The recombinant DNA, usually the cDNA sequence of the target protein, is designed to be under the control of a well characterized promoter and express the target protein within the chosen host cell to achieve high-level protein expression. Modification of the gene by recombinant DNA technology can lead to expression of a mutant protein or a large quantity of protein.
More about : Recombinant Protein and Its Expression Systems